Neurological Research and Animal Studies Laboratories
The Neurosurgery Department at Wayne State University has approximately 2000 square feet of laboratory space that is available for the SSIM program. Included are a 400 square foot histology lab, a 400 square foot cell/molecular lab, a 500 square foot small animal surgical suite, and two 200 square foot small animal behavioral testing labs. All labs are adjacent or near each other in the basement of the Lande Medical Research Building.
Three AAALAC-accredited facilities are available for small and large animal housing in the Lande building, Scott Hall, and the Elliman building. The Elliman building also contains state-of-the-art surgical facilities under the direction of a full-time veterinarian and two licensed surgical technologists.
An office and research facilities in the Neurosurgery Department include 2 Dell Pentium IV PCs, one of which is dedicated to the fluorescence microscopy morphometry system.
In addition to routine histological and immunocytochemical equipment, the following specialized systems are available:
Access to a Phillips 300 electron microscope maintained by the Department of Emergency Medicine.
- 3 Leica/Leitz light microscopes
- Leitz Macroscope
- Automated photography system
- Stereology program for light microscopy
- -80° freezer
- Paraffin embedding apparatus
- Rotary microtome for paraffin sections
- Laminar flow hood
- 3 –20oC freezers
- 3 refrigerators
- Liquid scintillation counter
- Morris water maze
- Roto-rod motor testing system
- Custom-made behavioral apparatus for motor learning testing
A state-of-the-art morphometry system coupled to a new Leica DMX epifluorescence upright microscope is also available. The morphometry system is the most recent
version developed by Microbrightfield, Inc. and includes the following components:
- 3D neuron reconstruction and analysis
- 3D anatomical brain mapping
- 3D visualization
- Automatic measurement of many parameters
- Serial section reconstruction
- Interactive image analysis
- Stereo Investigator
- Optical fractionator provides efficient, precise, unbiased and reliable estimates of the number and morphometric properties of biological structures
- Allows the operator to work directly with the light microscope, and with digital or hard-copy images from confocal microscopes, electron microscopes, and scanning topographic instruments
- Creates comprehensive maps of cells and anatomical regions
- Uses either the LucividTM for direct visualization and quantification through the microscopy or a video camera with frame grabber